AFRC MAC 7

Reactivity: Anti-Glycoprotein of Chlamydomonas reinhardii, 85102269, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514

Properties

• biological source: Rat x Rat Hybridoma
• packaging: tube of 5 μg 85102269-DNA-5UG; pkg of vial of cells 85102269-1VL
• ImmunoDon: AO rat spleen
• Myeloma: Y3Ag1.2.3
• Reactivity: Anti-Glycoprotein of Chlamydomonas reinhardii
• technique(s): cell culture | mammalian: suitable
• isotype: IgM
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Rat Y3Ag1.2.3 x Rat AO spleen

Cell Line Description

The antibody recognises the glycoprotein 2BII of Chlamydononas reinhardii. Binding is lost after acid hydrolysis or de-glycosylation of 2BII. AFRC MAC 7 shows similar specificity and properties to AFRC MAC 6, 8, 9, 10 and 11.

Immunogen

2BII, the major glycoprotein fraction of the cell wall of Chlamydomonas reinhardii

Culture Medium

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. These cells require mouse macrophage feeder layers upon resuscitation

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.