AFRC MAC 4
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., 85102266, Reactivity: Anti-Glycoprotein of Chlamydomonas reinhardii
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About This Item
UNSPSC Code:
41106514
biological source
Rat x Rat Hybridoma
packaging
tube of 5 μg 85102266-DNA-5UG
pkg of vial of cells 85102266-1VL
ImmunoDon
AO rat spleen
Myeloma
Y3Ag1.2.3
Reactivity
Anti-Glycoprotein of Chlamydomonas reinhardii
technique(s)
cell culture | mammalian: suitable
isotype
IgG2a
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Rat Y3Ag1.2.3 x Rat AO spleen
Cell Line Description
The antibody recognises the glycoprotein 2BII and labels both the whole cell surface and also the flagella. Binding is lost after acid hydrolysis or de-glycosylation of 2BII. AFRC MAC 4 shows similar specificity and properties to AFRC MAC 3 and 5.
Immunogen
2BII, the major glycoprotein fraction of the cell wall of Chlamydomonas reinhardii
Culture Medium
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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