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AFRC MAC 3

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., Reactivity: Anti-Glycoprotein of Chlamydomonas reinhardii, 85102265

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About This Item

UNSPSC Code:
41106514

biological source

Rat x Rat Hybridoma

packaging

tube of 5 μg 85102265-DNA-5UG
pkg of vial of cells 85102265-1VL

ImmunoDon

AO rat spleen

Myeloma

Y3Ag1.2.3

Reactivity

Anti-Glycoprotein of Chlamydomonas reinhardii

technique(s)

cell culture | mammalian: suitable

isotype

IgG2a

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Rat Y3Ag1.2.3 x Rat AO spleen

Cell Line Description

The antibody recognises the glycoprotein 2BII and labels both the whole cell surface and also the flagella. Binding is lost after acid hydrolysis or de-glycosylation of 2BII.

Immunogen

2BII, the major glycoprotein fraction of the cell wall of Chlamydomonas reinhardii

Culture Medium

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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