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H6/31HL

85102214, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., Reactivity: Anti-IgD, mouse

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About This Item

UNSPSC Code:
41106514
Pricing and availability is not currently available.

biological source

Mouse x Mouse Hybridoma

packaging

tube of 5 μg 85102214-DNA-5UG
pkg of vial of cells 85102214-1VL

ImmunoDon

BALB/c mouse spleen

Myeloma

P3X63Ag8

Reactivity

Anti-IgD, mouse

technique(s)

cell culture | mammalian: suitable

isotype

IgM

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Mouse P3X63Ag8 x Mouse BALB/c spleen

Cell Line Description

The antibody is directed against ′IgD-like′ molecules of mice with IgB haplotype. The target antigen has a molecular weight of 185kDa and is found on a high proportion of mature lymphoid cells, particularly on cells from Peyer′s patches. It is not detected on thymus cells.

Immunogen

Two grafts of full thickness skin from B10 mice followed by B10 spleen cells

Culture Medium

DMEM + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

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