RAMOS-AW

85062003, human lymph, Lymphoblast

• UNSPSC Code: 41106514

Properties

• Product Name: RAMOS-AW, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., 85062003
• biological source: human lymph
• packaging: tube of 5 μg 85062003-DNA-5UG; pkg of vial of cells 85062003-1VL
• growth mode: Suspension
• karyotype: Modal number 45 hypodiploid
• morphology: Lymphoblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human Caucasian Burkitt′s lymphoma

Cell Line Description

Derived by in vitro infection of Ramos cells (Sigma Catalogue number. 85030802) with EBV. The line is EBV positive. The cells have B lymphocyte characteristics, with surface associated mu and kappa chains.

Application

Tumourigenicity and virus studies: EBV

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 13,14
D16S539: 10,13
D5S818: 7,12
D7S820: 11
THO1: 7,9.3
TPOX: 8,9
vWA: 15,16

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.