H4-II-E-C3
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85061112, rat liver, Epithelial
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About This Item
UNSPSC Code:
41106514
Pricing and availability is not currently available.
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biological source
rat liver
packaging
tube of 5 μg 85061112-DNA-5UG
pkg of vial of cells 85061112-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Rat liver hepatoma
Cell Line Description
From H-35 Reuber hepatoma. Tyrosine aminotransferase positive. Synthesises several serum proteins including albumin, transferrin, prothrombin. The presence of type C virus has been shown and can readily be verified by electron microscopy.
Application
Enzymatic and virus studies: Type C virus
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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