RT112/84
85061106, human bladder, Epithelial
Synonym(s):
RT112-84 Cells
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About This Item
UNSPSC Code:
41106514
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Product Name
RT112/84, 85061106
biological source
human bladder
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human bladder carcinoma epithelial
Cell Line Description
A slow growing tumour which does not form colonies in soft agar. Tumorigenic in nude mice, RT1112/84 cells possess a low plating efficiency requiring 400 cells/dish to form any colonies.
Application
RT112/84 cancer cell line has been used to study the cytotoxic effect of lipoic acid and calcium hydroxycitrate concentrations on RT112/84 cells.[1]
Tumourigenicity studies
DNA Profile
STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 13,14
D16S539: 11,13
D5S818: 10,13
D7S820: 11,12
THO1: 7
TPOX: 8,11
vWA: 14,17
CSF1PO: 10,11
D13S317: 13,14
D16S539: 11,13
D5S818: 10,13
D7S820: 11,12
THO1: 7
TPOX: 8,11
vWA: 14,17
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) seeding at 2-4x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C. Subculture weekly - cells are difficult to trypsinise, and should be kept sub-confluent.
Other Notes
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Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
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