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product name
Phi-1, 85061101
biological source
rat liver
growth mode
Adherent
karyotype
hyperdiploid
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Rat Liver
Cell Line Description
Probably expresses other traits typical of H4-11-E derivatives. It is a derivative of an Fu5 derivative. HAT sensitive, HPRT-, resists 6μg/ml thioguanine. Highly differemtiated.
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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