biological source
Rat x Rat Hybridoma
ImmunoDon
AO rat spleen
Myeloma
YB2/3.0 Ag20
Reactivity
Anti-Phytochrome
technique(s)
cell culture | mammalian: suitable
isotype
IgG2a
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Rat YB2/3.0 Ag20 x Rat AO spleen
Cell Line Description
The antibody is directed against phytochrome from Avena. It fails to distinguish between the red-absorbing and far-red-absorbing forms of phytochrome.
Immunogen
Phytochrome, purified from dark-grown Avena Sativa l seedlings
Culture Medium
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation
Other Notes
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