Ethyl nitrosourea induced tumour cell line. Neuronal cells are useful for research into the distribution of neurotransmitter synthesis and brain specific antigens among nerve and glia. Cell morphology significantly affected when grown in serum free media.
Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
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