B95-8

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514

Properties

• biological source: cotton-top tamarin monkey blood
• packaging: tube of 5 μg 85011419-DNA-5UG; pkg of vial of cells 85011419-1VL
• growth mode: Suspension
• karyotype: Not specified
• morphology: Lymphoblast/Fibroblast
• products: Epstein Barr Virus (EBV)
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Cotton-top Tamarin Monkey peripheral blood lymphocyte

Cell Line Description

Derived from a cotton-top tamarin (Saguinus oedipus). Releases high titres of transforming Epstein Bar virus (EBV). Thus it provides a source of EBV to establish continuous lymphocytic lines from human donors. The cells should be handled under laboratory containment level 2.

In some instances the cell line B95-8 has been described as derived from ′marmoset′; however, this is not correct. B95-8 was derived from a cotton-top tamarin (Saguinas oedipus); this has been confirmed by DNA profiling.

Culture Medium

RPMI 1640 (R0883)+ 2 mM L-Glutamine (G7513) + 10% FBS / FCS (F2442).

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml. May need to use 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. It is recommended that this cell line be frozen at no less than 8x1000,000 cells per ampoule.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.