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product name
HF19, 85011418
biological source
human lung
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human foetal lung fibroblast
Cell Line Description
HF19 has been established from a 14 week old female foetus. The cells are heterozygous for thymidine kinase and have been used for studies of radiation-induced DNA damage.
Application
DNA repair studies
DNA Profile
STR-PCR Data: Amelogenin: X
CSF1PO: 10,13
D13S317: 9,13
D16S539: 13,14
D5S818: 11,12
D7S820: 11,12
THO1: 6,7
TPOX: 8,11
vWA: 14,18
CSF1PO: 10,13
D13S317: 9,13
D16S539: 13,14
D5S818: 11,12
D7S820: 11,12
THO1: 6,7
TPOX: 8,11
vWA: 14,18
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-4x10,000 cells /cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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