MCF7/ExeR-4

16022523, human breast, Polygonal epithelia

• UNSPSC Code: 41106514

Properties

• product name: MCF7/ExeR-4, from human breast, 16022523
• biological source: human breast
• growth mode: Adherent
• morphology: Polygonal epithelia
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

General description

Breast cancer cell line resistant to the aromatase inhibitor exemestane. Oestrogen receptor positive.

Cell Line Description

The MCF7/ExeR-4 Cell Line was developed as a model of resistance to anti-cancer treatment with aromatase inhibitors. Exemestane-resistant cell lines were established from MCF-7 cells grown in medium with 10% NCS and 10^-7 M testosterone. A culture of MCF-7 cells were treated with 10^-7 M exemetane for one week, trypsinized and seeded in serial dilutions in 24-well plates. Single colonies were transferred to new wells and gradually expanded in medium with exemestane. After ~2–3 months, the isolated colonies gave rise to exemestane-resistant cell lines, which could be grown in exemestane-containing medium with a weekly split ratio of ~1:25. The MCF-7 cell line was authenticated in January 2014 by DNA profiling using short tandem repeat loci and found to be matching the genetic profile reported for the MCF-7 cell line.

Culture Medium

Phenol red free DMEM:F12 (1:1) (D6434) + 10% Newborn Calf Serum(NBCS) (N4637) + 2.5 mM Ala-Gln (G8541) + 6 ng/ ml Insulin (I9278), 0.1 uM testosterone and 0.1 uM exemestane

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x 10, 000 cells/cm² using 0.05% trypsin or trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Citation

If use of this culture results in a scientific publication, it should be cited in the publication as: MCF7/ExeR-4 (ECACC 16022523)

Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.