MCF7/TAMR-7

16022509, human breast, Polygonal

• UNSPSC Code: 41106514

Properties

• Product Name: MCF7/TAMR-7, from human breast, 16022509
• biological source: human breast
• growth mode: Adherent
• morphology: Polygonal
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Description

The MCF7/TAMR-7 Cell line is a breast cancer cell line resistant to tamoxifen. Tamoxifen is by far the most widely used drug for hormone-dependent breast cancer. However, primary or acquired resistance to Tamoxifen severely limits its clinical effectiveness. The MCF7/TAMR-7 cell line is a good model cell to study the signalling pathways, which are the major drivers of tamoxifen-resistant growth.
The parental cell line for the MCF7/TAMR-7 cells is MCF7/S0.5, which was adapted to grow at low serum concentration in order to study the effect of estradiol and tamoxifen. MCF7/TAMR-7 has been established from a clone of cells that survived long term treatment with 1 μM tamoxifen. Tamoxifen-resistant cells are passaged continuously in presence of 1 μM tamoxifen, which is lethal for the parental MCF7/S0.5 cell line.

Culture Medium

Phenol red free DMEM:F12 (1:1) (D6434) + 1% FBS (F2442), 2.5 mM Ala-Gln (G8541) + 6 ng/ml insulin. To maintain high-level resistance, medium was supplemented with Tamoxifen (1 uM).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x 10, 000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C. Recommend media changes every 2-3 days.

Other Notes

Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Citation

If use of this culture results in a scientific publication, it should be cited in the publication as: MCF7/TAMR-7 (ECACC 16022509)

Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.