ULK2 KO MEF (SV40)

14050805, mouse embryo, Fibroblast

• UNSPSC Code: 41106514

Properties

• Product Name: ULK2 KO MEF (SV40), 14050805, from mouse embryo
• biological source: mouse embryo
• description: Mouse embryonic fibroblast, autophagy, Unc51-like kinase, SV40-immortalised
• growth mode: Adherent
• morphology: Fibroblast
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Description

Mouse embryonic fibroblasts (MEFs) derived from the ULK1(+/-) ULK2(+/-) knockout mice. MEFs were isolated and cultured and immortalised with SV40 large T-antigen. This cell line can be used to study ULK1 dependent processes in autophagy.

Culture Medium

DMEM + 10% FCS +2mM Glutamine

Subculture Routine

Trypsinize with 0.05% Trypsin/EDTA and inoculate new cultures at 2 x 10⁴ cells per cm². Cultures must be incubated in a humidified 5% CO₂/95% air incubator at 37^oC.

Other Notes

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Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.