MEF Ulk1 -/- Ulk2 -/- (DKO) (SIM)

14050803, mouse embryo, Fibroblast

• UNSPSC Code: 41106514

Properties

• product name: MEF Ulk1 -/- Ulk2 -/- (DKO) (SIM), from mouse embryo, 14050803
• biological source: mouse embryo
• description: Mouse embryonic fibroblast, autophagy, Ulk1 -/- Ulk2 -/-, double knockout, spontaneously immortalised
• growth mode: Adherent
• morphology: Fibroblast
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Description

Primary mouse embryonic fibroblasts (MEFs) were isolated from the embryos of a pregnant female Ulk1-/- Ulk2-/+ mouse at day 13.5 post coitus. The embryos were genotyped to identify those that were Ulk1 -/- Ulk2 -/- and the MEFs that were isolated and cultured were immortalised by a standard serial passaging protocol.

Culture Medium

DMEM + 10% FCS + 2mM Glutamine

Subculture Routine

Trypsinize with 0.05% Trypsin/EDTA and inoculate new cultures at 2 x 10,000 cells per cm². Cultures must be incubated in a humidified 5% CO₂/95% air incubator at 37^oC

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.