RN46A-B14
12061303, rat embryo d13 medullary raphe. Morphology: proliferating, fibroblast. Differentiating, neuronal.
Synonym(s):
46A B14, 46A-B14, RN46A B14
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About This Item
UNSPSC Code:
41106514
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product name
RN46A-B14, 12061303
biological source
rat embryo (day 13 medullary raphe)
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast morphology while proliferating and neuronal on differentiation
receptors
Not specified
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Cell Line Origin
Embryonic rat medullary raphe, temperature-sensitive mutant of SV40 large T-antigen, immortalised, serotonergic, neuronal
Cell Line Description
The clonal cell line, RN46A-B14, was isolated following transfection of the gene encoding rat brain-derived neurotrophic factor (BDNF) into RN46A cells (see ECACC Catalogue number 12061302). RN46A-B14 cells synthesize and secrete biologically active BDNF in vitro and synthesize serotonin (5-HT) following partial membrane depolarization. Two weeks following RN46A-B14 cell transplantation into the adult rat cortex and hippocampus, there is a threefold increase in survival of RN46A-B14 cells compared to RN46A cells. The grafted RN46A-B14 cells i mMunohistochemically stain for BDNF and 5-HT, while RN46A cells transfected with vector only are negative for both BDNF and 5HT. In addition, RN46A-B14 cells attain more morphologically complex phenotypes, indicating enhanced neuronal differentiation. Autocrine secretion of BDNF by RN46A-B14 cells thus potentiates survival and can be used to deliver both BDNF and 5-HT in vivo.
DNA Profile
Not specified
Culture Medium
DMEM:F12 (1:1) (D8062) + L-Glutamine (G7513) + 10% FBS / FCS (F2442) + 0.25mg/ml Geneticin (G418) + 0.1mg/ml hygromycin. Alternatively CNS medium can be used (see Kawamoto & Barrett 1986).
Subculture Routine
Split subconfluent cultures (70-80%) 1:2 to 1:5 using 0.25% trypsin/EDTA; 5% CO2; 33 °C. Suggested seeding density 2-4 x 10,000 cells/cm2. Doubling time 25hrs.
Other Notes
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Certificates of Analysis (COA)
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