C75
12022907, human colon, Epithelial
Synonym(s):
C 75, C-75
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About This Item
UNSPSC Code:
41106514
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Product Name
C75, from human colon, 12022907
biological source
human colon
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
Cell Line Origin
Human colorectal adenocarcinoma, moderately well differentiated, Dukes′ stage D
Cell Line Description
The C75 cell line was established from a 56-year old male patient with a moderately well differentiated adenocarcnoma of the sigmoid colon classified as Dukes′ stage D
DNA Profile
STR-PCR Data:
Amelogenin: X,Y
CSF1PO: 12
D13S317: 10,13
D16S539: 8,11
D5S818: 12
D7S820: 9,11
THO1: 8,9.3
TPOX: 10
vWA: 17
Amelogenin: X,Y
CSF1PO: 12
D13S317: 10,13
D16S539: 8,11
D5S818: 12
D7S820: 9,11
THO1: 8,9.3
TPOX: 10
vWA: 17
Culture Medium
Iscove′s Modified Dulbecco′s Medium, + 10% Foetal Bovine Serum (FBS) + 2 mM Glutamine
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37 °C. C75 cells grow very slowly (see growth curve); following resuscitation or subculture the cells take at least 48 hours.
Other Notes
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Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
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