C99
12022905, human colon, Epithelial
Synonym(s):
C 99, C-99
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
41106514
Pricing and availability is not currently available.
Recommended Products
Product Name
C99, from human colon, 12022905
biological source
human colon
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
Cell Line Origin
Human colorectal adenocarcinoma, moderately well differentiated, Dukes′ stage C
Cell Line Description
The C99 cell line was established from a 69-year old male patient with a moderately well differentiated adenocarcinoma of the rectum classified as Dukes′ stage C.
DNA Profile
STR-PCR Data:
Amelogenin: X
CSF1PO: 10,11
D13S317: 8,14
D16S539: 11,13
D5S818: 11,12
D7S820: 9,11
THO1: 8,9.3
TPOX: 8,10
vWA: 16,17
Amelogenin: X
CSF1PO: 10,11
D13S317: 8,14
D16S539: 11,13
D5S818: 11,12
D7S820: 9,11
THO1: 8,9.3
TPOX: 8,10
vWA: 16,17
Culture Medium
Iscove′s Modified Dulbecco′s Medium, + 10% Foetal Bovine Serum (FBS) + 2 mM Glutamine
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37 °C. C99 cells grow very slowly (see growth curve); following resuscitation or subculture the cells take at least 48 hours
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.
If you need assistance, please contact Customer Support.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
