C84 cell line human

12022902, human colon, Epithelial

• Synonym(s): C 84, C-84
• UNSPSC Code: 41106514

Properties

• product name: C84 cell line human, 12022902
• biological source: human colon
• growth mode: adherent
• morphology: Epithelial
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Description

The C84 cell line was established from a 67-year old male patient with a poorly differentiated adenocarcinoma of the caecum classified as Dukes′ stage C.

DNA Profile

STR-PCR Data:

Amelogenin: X
CSF1PO: 11,12
D13S317: 11,12
D16S539: 13
D5S818: 12
D7S820: 11
THO1: 7,9.3
TPOX: 8,9
vWA: 14,15

Culture Medium

Iscove′s Modified Dulbecco′s Medium, + 10% Fetal Bovine Serum (FBS) + 2 mM Glutamine

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x 10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

C84 cells grow very slowly; following resuscitation or subculture the cells take at least 48 hours to re-attach. Cells should be left without disturbance during this time to facilitate adhesion. Centrifugation of the cells (100g x 5 min) at resuscitation to remove DMSO improves the establishment of a viable culture. Once attached, the cells grow in discrete islands and use of trypsin or trypsin/EDTA to subculture the cells (even without knocking the flask) yields large clumps. Further disaggregation may be achieved by repeatedly pipetting the cells.

Other Notes

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Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.