ZL5

11120715, human lung (effusion), Spheroidal

• Synonym(s): ZL 5, ZL-5
• UNSPSC Code: 41106514

Properties

• Product Name: ZL5, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., from human lung(effusion), 11120715
• biological source: human lung (effusion)
• packaging: tube of 5 μg 11120715-DNA-5UG; pkg of vial of cells 11120715-1VL
• growth mode: Adherent
• karyotype: Modal
• morphology: Spheroidal
• products: Not specified
• receptors: Mesothelial-membrane antigen ME1
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice

Description

Cell Line Origin

A human mesothelioma cell line derived from a malignant mesothelioma. Asbestos exposure

Cell Line Description

ZL5 was derived from the pleural effusion of a 43-year old male patient, prior to treatment, with a known history of exposure to asbestos.

DNA Profile

STR-PCR Data:
Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 9,13
D16S539: 12
D5S818: 12
D7S820: 10,11
THO1: 6,9.3
TPOX: 8,11
vWA: 16,17

Culture Medium

DMEM:HAMS F12 (1:1) + 2mM Glutamine + 15% Foetal Bovine Serum (FBS)

Subculture Routine

Split subconfluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin/EDTA; 5% CO₂; 37 °C. Suggested seeding density 2-4x10,000 cells/cm^². The cells in suspension should be collected by centrifugation and added to the trypsinized cells before reseeding.

Other Notes

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