Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

K38 Keratinocyte Cell Line

11090602, mouse skin, Polygonal

Synonym(s):

K-38 Cells, K 38, K38, KSC, KSPC

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
41106514
Pricing and availability is not currently available.

Product Name

K38 Keratinocyte Cell Line, 11090602

biological source

mouse skin

growth mode

Adherent

karyotype

Not specified

morphology

Polygonal

receptors

Not specified

Looking for similar products? Visit Product Comparison Guide

Cell Line Origin

Murine keratinocyte stem cell line

Cell Line Description

Keratinocytes were isolated from neonatal wild type BALB/c mouse skin. The cell line was established by growing cells for the first 8 passages in co-culture with 3T3 J2 fibroblast feeder cells. From passage 9 onwards cells were grown without feeder cells. This line can be used for the generation of in vitro epidermal skin equivalents to be used as screening tools to look at the activity of NCEs and/or NBEs of dermatological interest, and/or to screen for such NCE and/or NBE in high throughput systems. It may also be of use to non pharmaceutical healthcare and/or cosmetic companies involved in the personal vitality and/or beauty product development segments. The spontaneously immortalized keratinocytes can be differentiated by the addition of calcium to the complete FAD medium at a final concentration of 1.2 mM (high calcium medium). Confluent cultures stratify when grown in high calcium medium.

DNA Profile

Not specified

Culture Medium

The cell line is grown in low calcium medium, DMEM/Ham′s F12 with FBS / FCS (F2442) and several supplements at 32 °C, 5% CO2. The supplements are: 0.18 mM adenine, 0.5 μg/ml hydrocortisone, 5 μg/ml insulin,10 -10 M cholera toxin, 10 ng/ml EGF, 2 mM L-Glutamine (G7513), 1 mM pyruvate.

Subculture Routine

Wash monolayer with PBS (without Ca2+ and Mg2+), incubate with EDTA solution for 5 min at room temperature and finally incubate for 8-12 min with 0.05% trypsin/0.02% EDTA at 37 °C until they come off easily. Add complete FAD medium and resuspend the cells by pipetting up and down 3-5 times. After centrifugation for 5 min at 220 g, resuspend the pellet in complete FAD medium, and seed the cells onto collagen I-coated dishes. Seed new cultures at 3-5 x 104 cells/cm2.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Questions

  1. 11090602-DNA-5UG For this product, is only the cell line ordered or the culture medium as well comes along with it. From the description it's not clear. If not, please provide SKU for the relative culture medium.

    1 answer

    1. This product is only the DNA of the K38 Keratinocyte cell line. The product number for the frozen cell line is 11090602-1VL. A prepared medium for use in culturing these cells is not offered. The supplier (ECACC) recommends culturing in low calcium medium, DMEM/Ham′s F12 with FBS / FCS (F2442) and several supplements at 32 °C, 5% CO2. The supplements are: 0.18 mM adenine, 0.5 μg/ml hydrocortisone, 5 μg/ml insulin,10 -10 M cholera toxin, 10 ng/ml EGF, 2 mM L-Glutamine (G7513), 1 mM pyruvate. To assure maximum expansion and overall health of the cell line, adhering to this formulation is highly recommended.

      For further information on this product, please view the ECACC website: https://www.culturecollections.org.uk/products/celllines/generalcell/detail.jsp?refId=11090602&collection=ecacc_gc

      Helpful?

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service