BRIN-BG5, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., 10033001
biological source
rat pancreas (islets)
packaging
tube of 5 μg 10033001-DNA-5UG pkg of vial of cells 10033001-1VL
growth mode
Adherent
karyotype
Modal chromosome number 66 up to passage 50
morphology
Epitheloid
products
BRIN-BG5 is a stable, glucose-responsive insulin secreting beta cell line expressing insulin, glucokinase and IAPP as detected by immunocytochemistry. BRIN-BG5 cells express the GLUT2 glucose transporter.
A rat pancreatic Beta cell: RINm5F hybrid cell line. Insulin-secreting cell line
Cell Line Description
BRIN-BG5 is a hybrid cell line formed by the electrofusion of a primary culture of NEDH rat pancreatic islets with RINm5F (a cell line derived from a NEDH rat insuliinoma). BRIN-BG5 has been shown to be tumourigenic when transplanted into a SCID mouse host. BRIN-BG5 has been mycoplasma eradicated at ECACC and the stocks available for supply have undergone a further 10 passages without detection of mycoplasma.
Split sub-confluent cultures (70-80%) 1:8 to 1:10, i.e. seeding at 2-4 x 104 cells/cm2 using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37 °C. Population doubling approximately28hrs. At confluence 105 cells/cm2 can be expected.
Other Notes
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Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
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Certificates of Analysis (COA)
Lot/Batch Number
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