All mammalian cell lines offered by ECACC are certified mycoplasma-free. No mycoplasma DNA was detected in PCR testing using validated mycoplasma-specific primers.
MKL-1
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 09111801, human skin. Loose floating aggregates with irregular outline and no central necrosis.
Synonym(s):
MKL1 Cells
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About This Item
UNSPSC Code:
41106514
Pricing and availability is not currently available.
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biological source
human skin
packaging
tube of 5 μg 09111801-DNA-5UG
pkg of vial of cells 09111801-1VL
growth mode
Suspension
karyotype
Hyperdiploid
morphology
Loosely packed floating aggregates with irregular outline and no central necrosis
products
MKL-1 is positive for CM2B4/MCV T AE1/AE2, CK20, EMA, CAM5.2, NSE and TTF-1. MKL-1 is negative for CK7, chromagen, synaptophysin, neurofilament, LCA, vimentin and CD99.
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
metastasis
shipped in
dry ice
Cell Line Origin
Human neuroendocrine skin carcinoma
Cell Line Description
MKL-1 was established from a nodal metastasis in a 26-year-old Caucasian male patient. MKL-1 cells contain a Merkel cell polyomavirus integrated into the genome. MKL-1 cells cause tumour formation at 4-6 weeks at subcutaneous sites in athymic male nude mice (BALB/c nu/nu).
DNA Profile
STR-PCR Data:
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,11
D16S539: 10,12
D5S818: 11,12
D7S820: 8,11
THO1: 9,9.3
TPOX: 8
vWA: 16,18
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,11
D16S539: 10,12
D5S818: 11,12
D7S820: 8,11
THO1: 9,9.3
TPOX: 8
vWA: 16,18
Culture Medium
RPMI 1640 + 2 mM Glutamine + 10% FBS
Subculture Routine
Seed at 3 x 105 cells/ml in 5% CO2; 37 °C Saturation density is approximately 106 cells/ml. Population doubling time is around 120 hours.
Other Notes
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Have the cells been certified as mycoplasma-free?
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Is there a method to address the clumping of cells, as it is difficult to count them individually and obtain an accurate cell concentration?
1 answer-
As per the European Collection of Authenticated Cell Cultures (ECACC), this cell line is recognized for growing as irregularly outlined floating aggregates, which can vary from chain form to spherical in appearance. Achieving single-cell suspension is challenging due to the aggregate nature. In the literature, it is commonly suggested to use 1x Accutase to break up cell clumps and achieve single-cell suspension.
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