UM-UC-4

8090501, human lymph, Epithelial

• UNSPSC Code: 41106514

Properties

• Product Name: UM-UC-4, 08090501
• biological source: human lymph
• growth mode: Adherent
• karyotype: Modal number of chromosomes is 51
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): metastasis
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human adenocarcinoma lymph node metastasis

Cell Line Description

UM-UC-4 was obtained from an open operative specimen from a metastatic tumour of the lymph node; the female patient had an urachal carcinoma. UM-UC-4 was found to produce tumours in athymic nude mice.

DNA Profile

STR-PCR Data:
Amelogenin: X
CSF1PO: 11
D13S317: 9
D16S539: 13
D5S818: 11
D7S820: 9,13
THO1: 9.3
TPOX: 8
vWA: 17,19

Culture Medium

EMEM (EBSS) + 2 mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:8, i.e., seeding at 2-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37 °C. Doubling time approximately 32 hours.

Other Notes

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Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.