N18 (Hamprecht)
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
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About This Item
UNSPSC Code:
41106514
Pricing and availability is not currently available.
biological source
mouse nerve
packaging
tube of 5 μg 08062527-DNA-5UG
pkg of vial of cells 08062527-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Not specified
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse neuroblastoma
Cell Line Description
N18 is a clonal line produced from the mouse neuroblastoma C1300. (Mouse strain A/Jax). ECACC has two catalogue entries for the N18 cell line which differ in that they were deposited by different groups: N18 from the original group that derived the cell line (Sigma Catalogue number 88112301) and N18(Hamprecht) (Sigma Catalogue number. 08062527). Several other cell lines in the ECACC catalogue have been derived from N18(Hamprecht).
Application
Can be used as a neuronal model
Culture Medium
DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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