NH15-CA2

8062525, Mouse x Rat Glioma Hybrid brain, Neuronal

• UNSPSC Code: 41106514

Properties

• Product Name: NH15-CA2, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., 08062525
• biological source: Mouse x Rat Glioma Hybrid brain
• packaging: tube of 5 μg 08062525-DNA-5UG; pkg of vial of cells 08062525-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Neuronal
• products: Acetylcholine and noradrenaline
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• Storage temp.: −196°C

Description

Cell Line Origin

Mouse neuroblastoma x rat glioma hybrid

Cell Line Description

NH15-CA2 is a hybrid cell line formed by the fusion of N115-BU-8 (a bromodeoxyuridine-resistant mutant of N1E-115 (Sigma Catalogue number 08062519) and 108CC15 (Sigma Catalogue number 08062516) (itself a mouse neuroblastoma N18TG2 x rat glioma C6-BU-1 hybrid line). This “hybrid-hybrid” line contains both cholinergic and adrenergic enzyme activities.

Application

used as a neuronal model

Culture Medium

DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) HAT (0.1 mM hypoxanthine, 10 μM aminopterin and 16 μM thymidine) Cells can be cultured without HAT provided they were cultured inbetween in HT medium for 3-4 days

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-2x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.