N18TG2, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., 08062523
biological source
mouse nerve
packaging
tube of 5 μg 08062523-DNA-5UG pkg of vial of cells 08062523-1VL
A 6-thioguanine-resistant clone of N18 (a clonal line produced from the mouse neuroblastoma C1300). (Mouse strain A/Jax). This cell line exhibits high levels of acetylcholinesterase. Found to be tumourigenic in BALB/c nude mice.
application
Used in the generation of cell hybrids.
Culture Medium
DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + 0.1 mM 6-thioguanine
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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