B82 (Hamprecht)

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514

Properties

• biological source: mouse connective tissue
• packaging: tube of 5 μg 08062522-DNA-5UG; pkg of vial of cells 08062522-1VL
• growth mode: Adherent
• karyotype: Modal No of chromosomes is 53
• morphology: Fibroblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Mouse Fibroblast (L cells)

Cell Line Description

An 8-bromodeoxyuridine resistant mutant of mouse L cell fibroblasts (Mouse strain C3H/An). B82 lacks thymidine kinase and was isolated by virtue of resistance to 8-bromodeoxyuridine (30 μg/ml). ECACC has two catalogue entries for the B82 cell line which differ in that they were deposited by different groups: B82 (Sigma Catalogue number 85011408) and B82(Hamprecht) (Sigma Catalogue number 08062522). Several other cell lines in the ECACC catalogue have been derived from B82(Hamprecht).

Application

Used in the generation of cell hybrids.

Culture Medium

DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + 1 mM 8-bromodeoxyuridine

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.