N1E-115-1

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 8062511, mouse nerve, neuronal

• UNSPSC Code: 41106514

Properties

• biological source: mouse nerve
• packaging: tube of 5 μg 08062511-DNA-5UG; pkg of vial of cells 08062511-1VL
• growth mode: Adherent
• karyotype: modal no. of chromosomes is 192 see reference
• morphology: neuronal
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Mouse neuroblastoma

Cell Line Description

A subclone of the cell line N1E-115 established by Amano et al. The cell line N1E-115 is an adrenergic cell line derived from mouse neuroblastoma C1300. (Mouse strain A/Jax). N1E-115 cells are reported to be slightly contaminated with with a cell type of the morphology of C6 glioma cells. It is recommended to use N1E-115-1, a subclone of N1E-115, that is devoid of the contamination.

Application

can be used as a neuronal model

Culture Medium

DMEM + 2 mM Glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C. Population doubling approximately 20 hours.

Other Notes

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