PNT1A (SERUM FREE)

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 7052901, human prostate, Not specified

• UNSPSC Code: 41106514

Properties

• biological source: human prostate
• packaging: tube of 5 μg 07052901-DNA-5UG; pkg of vial of cells 07052901-1VL
• growth mode: Semi-adherent
• karyotype: Not specified
• morphology: Not specified
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human post pubertal normal, immortalised with SV40, serum-free

Cell Line Description

This cell line is a derivative of the normal human prostate cell line PNT1A (Sigma cat no. 95012614) adapted to grow in serum-free medium. PNT1A was established by immortalisation of normal adult prostatic epithelial cells by transfection with a plasmid containing SV40 genome with a defective replication origin. The primary culture was obtained from the prostate of a 35 year old male at post mortem.

Culture Medium

Serum-free Stemline Keratinocyte Medium II (Sigma cat no. S0196) + Keratinocyte Medium Supplement (Sigma cat no. K3136). To freeze cells use 50:50 fresh:conditioned medium + 10% DMSO

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2; 5% CO₂; 37 ºC. Mixture of attached and suspension cells. To harvest attached cells use gentle agitation. If cells do not detach, then try using 0.25% trypsin. A trypsin inhibitor must be used to inactivate trypsin. Seed contents of one ampoule into a 25 cm² flask.

Other Notes

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