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KARPAS 231

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 06120601, human blood (leukemia), Single round to polymorphic cells growing in suspension

Synonym(s):

KARPAS, 231

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About This Item

UNSPSC Code:
41106514
Pricing and availability is not currently available.

biological source

human blood (leukemia)

packaging

tube of 5 μg 06120601-DNA-5UG
pkg of vial of cells 06120601-1VL

growth mode

Suspension

karyotype

Not specified

morphology

Single round to polymorphic cells growing in suspension

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

Cell Line Origin

Human B-cell leukemia

Cell Line Description

Established from peripheral blood of a 71 year-old woman at diagnosis of B-cell acute lymphoblastic leukemia (ALL). No rearrangement of C-MYC DNA sequences.

DNA Profile

Not specified

Culture Medium

RPMI 1640 + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 0.5 - 2 x1000,000 cells/ml; 5% CO2; 37 °C. Doubling time approximately 60 hours. Split saturated culture 1:2 every 2-4 days. Cells are single round to polymorphic and grow in suspension.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

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Certificates of Analysis (COA)

Lot/Batch Number

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Questions

  1. Hi, Is Karpas 422 mammalian cells or DNA extracted from a mammalian cell line? Thanks

    1 answer
    1. This product 06120601 is Karpas -231 mammalian cells. Product 06101702 is karpas 422 mammalian cells. For each of these products (and most ECACC products) the SKU designation following the product number identifies the specific product available to order. In the case of 06120601, the -1VL SKU will be the actual frozen cell line stock. The -DNA-5UG will be 5ug of DNA extracted from this cell line. The same is true for -RNA-5UG and -CDNA-20UL.

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