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Product Name
H413, 06092007
biological source
human mouth
growth mode
Adherent
karyotype
Not specified
morphology
Polygonal
products
Not specified
receptors
TGFbeta type I receptor, TGFbeta II receptor, TGFbeta III receptor
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human oral squamous cell carcinoma, buccal mucosa
Cell Line Description
Established from a squamous cell carcinoma (SCC) of the buccal mucosa (20mm-40mm) of a 53 year-old female patient. STNMP stage II, moderately differentiated, node negative tumour. Mutant p53, codon 68 exon 4, G to T; wild type K-, N- and Ha-ras. These cells are non-tumourigenic on subcutaneous injection into athymic nude mice, but tumourigenic on injection into the floor of the mouth.
DNA Profile
STR-PCR Data:
Amelogenin: X
CSF1PO: 10
D13S317: 12
D16S539: 11
D5S818: 12
D7S820: 9,10
THO1: 6,9.3
TPOX: 8,11
vWA: 16,20
Amelogenin: X
CSF1PO: 10
D13S317: 12
D16S539: 11
D5S818: 12
D7S820: 9,10
THO1: 6,9.3
TPOX: 8,11
vWA: 16,20
Culture Medium
DMEM:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.5 ug/ml sodium hydrocortisone succinate
Subculture Routine
Split sub-confluent cultures (70-80%), approximately every 5-6 days, 1:8 to 1:10 using 0.05% trypsin/EDTA; 5% CO2; 37°C. Suggested seeding density 5 x 1000 cells/cm2. Cells can take approximately 10 minutes to detach, an alternative is to trypsinise 2 to 3 times with fresh trypsin for shorter periods for each trypsin application. Avoid knocking flasks during the trypsinisation process as this can lead to loss of viability.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
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