H314
6092003, human mouth (floor), Polygonal & spindle
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About This Item
UNSPSC Code:
41106514
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Product Name
H314, 06092003
biological source
human mouth (floor)
growth mode
Adherent
karyotype
Not specified
morphology
Polygonal & spindle
products
Not specified
receptors
TGFbeta type I receptor, TGFbeta II receptor, TGFbeta III receptor
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human oral squamous cell carcinoma, floor of mouth
Cell Line Description
Established from a squamous cell carcinoma (SCC) of the floor of the mouth (20mm-40mm) of an 82 year-old male patient. STNMP stage II, moderately differentiated, node positive tumour. Mutant p53, codon 176 exon 5, G to T and 373 exon 11, A to G; wild type K-, N- and Ha-ras. Tumourigenic in athymic nude mice on subcutaneous injection; on injection into the floor of the mouth, cells spread to lymphatics and lymph nodes. By short tandem repeat (STR)-PCR analysis the Y chromosome could not be detected in this cell line when tested at ECACC. It is a known phenomenon that SSC cell lines can lose their Y chromosome.
Culture Medium
DMEM:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.5 ug/ml sodium hydrocortisone succinate
Subculture Routine
Split sub-confluent cultures (70-80%), approximately every 6-7 days, 1:8 to 1:10 using 0.05% trypsin/EDTA; 5% CO2; 37°C. Suggested seeding density 7 x 1000 cells/cm2.
Other Notes
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Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
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