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Key Documents

UM-UC-1

6080301, human kidney (renal gland), Epithelial

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About This Item

UNSPSC Code:
41106514
Pricing and availability is not currently available.

Product Name

UM-UC-1, 06080301

biological source

human kidney (renal gland)

growth mode

Adherent

karyotype

Not specified

morphology

Epithelial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

metastasis

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Human bladder transitional cell carcinoma

Cell Line Description

Human transitional cell carcinoma (grade II) derived from a bladder cancer metastasis of a black male. Metastatic involvement of lymphatics. By short tandem repeat (STR)-PCR analysis the Y chromosome could not be detected in this cell line when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection.

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 12,14
D16S539: 9,11
D5S818: 13
D7S820: 8,11
THO1: 8
TPOX: 8
vWA: 14,15

Culture Medium

MEM + 1% NEAA + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split subconfluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 2-4x10,000 cells/cm2 using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. Doubling time approximately 26 hours.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

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