KARPAS 45

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514

Properties

• biological source: human bone marrow
• packaging: tube of 5 μg 06072602-DNA-5UG; pkg of vial of cells 06072602-1VL
• growth mode: Suspension
• karyotype: Hypotetraploid
• morphology: Spherical cells
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human T-cell Leukaemia

Cell Line Description

A human T cell leukaemia cell line established from the bone marrow of a 2-year-old boy diagnosed with acute lymphoblastic leukaemia (ALL) prior to therapy in 1972; cells were reported to carry the MLL-AFX and AFX-MLL fusion genes, the original diagnosis was T-ALL.

Culture Medium

RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS). For slower growth use 10% or 5% FBS.

Subculture Routine

Maintain cultures between 0.5 - 2 x1000,000 cells/ml; 5% CO₂; 37°C. Doubling time 50-100 hours. Split saturated culture 1:2 to 1:3 every 2-3 days. Spherical cells growing in clusters in suspension.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.