AVG-16
6062701, pig ovary (follicle), Fibroblast
Synonym(s):
AVG 16, AVG(16), AVG16
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About This Item
UNSPSC Code:
41106514
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Product Name
AVG-16, 06062701, from pig ovary(follicle)
biological source
pig ovary (follicle)
growth mode
Not specified
karyotype
2n=38
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
Cell Line Origin
Porcine Reversibly Immortalised Primary Granulosa Cells
Cell Line Description
AVG-16 consists of reversibly immortalised primary granulosa cells. Primary cultures of pig granulosa cells have been cultured in the presence of the media supplement Ovagrow-S2A resulting in the establishment of an immortalised porcine primary granulosa cell line. Ovagrow-S2A, produced by Aivogen, comprises a naturally derived complex of growth promoting compounds, carrier proteins, and factors. These cells are not contact inhibited. The cells are conditionally immortalised; cell death will occur in the absence of OvagrowS2A and/or fibroblast growth factor-basic (bFGF).
DNA Profile
Not specified
Culture Medium
DMEM + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS) + 1% NEAA + 2.5ng/ml Fibroblast Growth Factor-Basic (bFGF) + 4% OvagrowS2A
Subculture Routine
Split subconfluent cultures (70-80%) 1:5 to 1:10, i.e., seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C. Saturation density: 1.5x100,000 cells/cm2.
Other Notes
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Legal Information
Patent publication No is WO2005021737
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