BICR 3
6032203, human mouth, Epithelial, keratinocyte
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About This Item
UNSPSC Code:
41106514
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Product Name
BICR 3, 06032203
biological source
human mouth
growth mode
Adherent
karyotype
Aneuploid, Modal no. 49
morphology
Epithelial, keratinocyte
products
Not specified
receptors
Epidermal Growth Factor Receptor (EGFR)
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Alveolus squamous cell carcinoma
Cell Line Description
Adherent keratinocyte cell line derived from a squamous cell carcinoma of the alveolus of a Caucasian female. Keratin and involucrin markers present. Known mutations: p53 and p16. Cells are tumourigenic in athymic mice. Epidermal Growth Factor Receptor (EGFR) present.
Culture Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.4 micrograms/ml Hydrocortisone.
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 using 0.05% trypsin/EDTA; 8% CO2; 37 C. Suggested seeding density 4 x 10,000 cells/cm2. A feeder layer of lethally-irradiated or mitomycin C-treated 3T3 feeder cells can be used to culture this cell line, howeve
Other Notes
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Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
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