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Key Documents

BICR 56

6031002, human mouth, Epithelial, keratinocyte

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About This Item

UNSPSC Code:
41106514
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Product Name

BICR 56, 06031002

biological source

human mouth

growth mode

Adherent

karyotype

Aneuploid, Modal no. 86

morphology

Epithelial, keratinocyte

products

Not specified

receptors

Epidermal Growth Factor Receptor (EGFR)

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Tongue squamous cell carcinoma

Cell Line Description

Adherent keratinocyte cell line derived from a squamous cell carcinoma of the tongue of a Caucasian female. Keratin and involucrin markers present. Known mutations: p53 and p16. Cells are tumourigenic in athymic mice. Epidermal Growth Factor Receptor (EGFR) present.

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.4 micrograms/ml Hydrocortisone.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 - 1:4 using 0.05% trypsin/EDTA; 8% CO2; 37 C. Suggested seeding density 4 x 10,000 cells/cm2. A feeder layer of lethally-irradiated or mitomycin C-treated 3T3 feeder cells can be used to culture this cell line,

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

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