FTC-236

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 6030202, human thyroid (lymph node metastasis), Endothelial

• UNSPSC Code: 41106514

Properties

• biological source: human thyroid (lymph node metastasis)
• packaging: tube of 5 μg 06030202-DNA-5UG; pkg of vial of cells 06030202-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Endothelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): metastasis
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human Thyroid Cancer Neck Lymph Node Metastasis

Cell Line Description

Derived from a neck lymph node metastasis of a follicular thyroid cancer (FTC).This cell line is tumourigenic in Balb/c mice. FTC-133 (Sigma Catalogue number 94060901), FTC-236 (Sigma Catalogue number 06030202) and FTC-238 (Sigma Catalogue number 94060902) were all derived from the same individual, a 42 year old male with follicular thyroid cancer. This has been confirmed by short tandem repeat STR-PCR analysis at ECACC The Y chromosome could not be detected in these cell line by (STR)-PCR analysis. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell lines are identical to the source provided by the depositor based on the STR-PCR analysis.

Culture Medium

DMEM and Ham′s F12 (1:1 mixture) + 10ug/ml Insulin + 0.01U/ml Thyroid Stimulating Hormone (TSH) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

Other Notes

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