BICR 6
5070501, human pharynx, Epithelial, keratinocyte
Synonym(s):
BICR 6 Cell Line, BICR 6 Human Cells, Human BICR 6
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About This Item
UNSPSC Code:
41106514
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Product Name
BICR 6, 05070501
biological source
human pharynx
growth mode
Adherent
karyotype
Aneuploid
morphology
Epithelial, keratinocyte
products
Not specified
receptors
Epidermal Growth Factor Receptor (EGFR)
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Hypopharynx squamous cell carcinoma
Cell Line Description
Adherent keratinocyte cell line derived from a squamous cell carcinoma of the hypopharynx of a Caucasian male. Keratin and involucrin markers present. Known mutations: p53, p16 and p14ARF. Cells are tumourigenic in athymic mice. Epidermal Growth Factor Receptor (EGFR) present.
Culture Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.4 micrograms/ml Hydrocortisone.
Subculture Routine
Split sub-confluent cultures (70-80%) 1:10 - 1:50 using 0.05% trypsin/EDTA; 8% CO2; 37 C. Suggested seeding density 2 x 10,000 cells/cm2. Culture cells on a feeder layer of lethally-irradiated or mitomycin C-treated 3T3 feeder cells.
Other Notes
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Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
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