RaBE

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514

Properties

• biological source: rat lung
• packaging: tube of 5 μg 04100601-DNA-5UG; pkg of vial of cells 04100601-1VL
• growth mode: Adherent
• karyotype: Hypotetraploid, n=74 chromosomes (median)
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Rat Bronchial Epithelial Cells (RaBE)

Cell Line Description

Spontaneously immortalised rat bronchial epithelial cell line derived from a 4 month old male Wistar rat. The cell line is basal cell-like expressing cytokeratins 14 and 15. Vimentin negative. A slow growing derivative of RaBE, cultured in the absence of growth factors is also available - RaBE (Slow Growth), Sigma Catalogue number. 04100602.

Culture Medium

Ham′s F12 + 2mM Glutamine + 10ug/ml transferrin + 5ug/ml insulin + 10ng/ml EGF + 10ng/ml aFGF + 100ng/ml cholera toxin + 5% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) between 1:100 and 1:800 i.e. seeding 5000 cells/25cm² with 5ml or 10,000 cells/75cm² with 20ml using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Subculture once a week, double volume

Other Notes

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