Rolf B1.T

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514

Properties

• biological source: rat nose
• packaging: tube of 5 μg 03071601-DNA-5UG; pkg of vial of cells 03071601-1VL
• growth mode: Adherent
• karyotype: Normal rat chromosome complement
• morphology: Epithelial-like
• products: cadherin, tenascin, laminin, N-CAM, GFAP, p75 NTR
• receptors: p75 NTR
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Adult rat olfactory nerve ensheathing cells

Cell Line Description

Rolf B1.T cells arose from a spontaneous immortalisation event - retaining characteristics of normal cells. The cells are dependent on serum growth factors for growth. They constitutively express glial fibrillary acidic protein, S100, the low affinity neurotrophin receptor p75 NTR, laminin, tenascin, and NCAM. These cells support the regrowth of neurites from adult rat retinal ganglion cells. They have potential value in research into mechanisms of glial support for axonal regeneration from adult mammalian central neurons and other aspects of differentiated glial cell biology. Epithelial-like or fusiform (inducible).

Application

Neural research including axonal regeneration.

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Split sub-confluent cultures (70-80%) 1:5 - 1:20 e.g. seeding at 2-4 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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