Recommended Products
product name
MOCHA, 02111901
biological source
mouse skin
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse Skin Fibroblast
Cell Line Description
Mocha is a naturally occuring mouse mutant with a null mutation in the delta subunit of AP-3 complex. In Mocha cells, the ß3 amd μ3 subunits coassmeble into a heterodimer whereas the sigma 3 subunit remains monomeric. The mouse has a similar phenotype to the human genetic disorder, Hermansky Pudlak Syndrome (HPS). In both mice and humans, AP-3 deficiency results in hypopigmentation because of abnormalities in melanosomes. The Mocha cell line can be used to investigate the assembly of AP-3 complexes and AP-3 function.
Culture Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 5x1,000 to 1x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service