C9998

Anti-CSTF3 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-CF-1 77 kDa subunit, Anti-CSTF-77, Anti-Cleavage stimulation factor 77
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~77 kDa
• species reactivity: human
• concentration: ~1.0 mg/mL
• technique(s): immunoprecipitation (IP): 2.5-5 μg using lysates of HEK-293T cells; indirect immunofluorescence: 1.25-2.5 μg/mL using paraformaldehyde fixed HeLa cells; western blot: 0.5-1 μg/mL using lysates of HEK-293T cells
• UniProt accession no.: Q12996
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... CSTF3(1479) ; mouse ... Cstf3(228410)

Description

General description

CstF is a heterodimer consisting of subunits of 50, 64 and 77 kDa referred to as CSTF1, CSTF2 and CSTF3, respectively. CSTF3 contains 12 HAT repeats followed by a proline-rich segment. CSTF3 also interacts directly with the RNA polymerase II CTD.

Application

Anti-CSTF3 (C-terminal) antibody produced in rabbit is suitable for immunoprecipitation at a working concentration of 2.5-5 μg using lysates of HEK-293T cells, indirect immunofluorescence at 1.25-2.5 μg/mL using paraformaldehyde fixed HeLa cells and western blotting at 0.5-1 μg/mL using lysates of HEK-293T cells.

Biochem/physiol Actions

The cleavage stimulatory factor (CstF) is a part of the mammalian mRNA 3′ end processing complex and interacts with a less-conserved G/U-rich sequence situated downstream of the cleavage site. CSTF3 bridges CSTF1 and CSTF2 during the assembly of the CstF complex and is required for proper 3′-end cleavage.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable