C9602
Anti-Chloride Channel-3 (CLC-3, Clcn3) antibody produced in rabbit
affinity isolated antibody, lyophilized powder
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About This Item
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized powder
species reactivity
rat
technique(s)
western blot (chemiluminescent): 1:200-1:300 using rat heart membranes and ECL.
UniProt accession no.
storage temp.
−20°C
Gene Information
rat ... Clcn3(84360)
General description
The protein contains a chloride channel (ClC) domain and two additional C-terminal CBS (cystathionine beta-synthase) domains. The ClC domain catalyzes the selective flow of Cl- ions across cell membranes, and the CBS domain may have a regulatory function.
Specificity
The epitope has significant homology with CLC-4 and CLC-5 proteins in rat and is well conserved in vertabrates.
Immunogen
highly purified GST fusion protein of the rat chloride channel-3 (CLC-3) corresponding to amino acids 592-661.
Application
Anti-Chloride Channel-3 (CLC-3, Clcn3) antibody produced in rabbit is suitable for western blotting (chemiluminescent) at a working dilution of 1:200-1:300 using rat heart membranes and ECL. It was used as a primary antibody for western blotting at a dilution of 1:2000 to demonstrate adenoviral-mediated overexpression of ClC-3.
Biochem/physiol Actions
The encoded protein, voltage-gated chloride channel, is a transmembrane protein that maintains chloride ion homeostasis in various cells. It contains a chloride channel (ClC) domain and two additional C-terminal CBS (cystathionine beta-synthase) domains. The ClC domain catalyzes the selective flow of Cl- ions across cell membranes, and the CBS domain may have a regulatory function. ClC-3 deficiency inhibits Ang II-induced apoptosis of endothelial progenitor cells via suppressing ROS (reactive oxygen species) generation derived from NADPH oxidase. Osmotically active Cl- is extruded via this channel upon kinase activation, which facilitates cytoplasmic condensation preceding cell division. ClC-3 protein may be considered as a potential tumor marker and therapeutic target for human nasopharyngeal carcinoma.
Physical form
Lyophilized at ~0.3 mg/ml from phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, 5% sucrose, and 0.025% sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1
Storage Class
13 - Non Combustible Solids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Jing Liu et al.
Apoptosis : an international journal on programmed cell death, 18(10), 1262-1273 (2013-07-23)
Endothelial progenitor cells (EPCs) play an important role in postnatal neovascularization and re-endothelialization in response to tissue ischemia and endothelial injury. It is reported that the circulating EPCs number is decreased during hypertension. However, the detailed mechanism is still unclear.
Maddalena Comini et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 42(15), 3080-3095 (2022-03-05)
ClC-3, ClC-4, and ClC-5 are electrogenic chloride/proton exchangers that can be found in endosomal compartments of mammalian cells. Although the association with genetic diseases and the severe phenotype of knock-out animals illustrate their physiological importance, the cellular functions of these
Vishnu Anand Cuddapah et al.
American journal of physiology. Cell physiology, 302(3), C527-C538 (2011-11-04)
"Mitotic cell rounding" describes the rounding of mammalian cells before dividing into two daughter cells. This shape change requires coordinated cytoskeletal contraction and changes in osmotic pressure. While considerable research has been devoted to understanding mechanisms underlying cytoskeletal contraction, little
Takehiko Ogura et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 16(8), 863-865 (2002-04-23)
We have cloned ClC-3B, a novel alternative splicing variant of ClC-3 (ClC-3A) that is expressed predominantly in epithelial cells. ClC-3B has a different, slightly longer C-terminal end than ClC-3A and contains a consensus motif for binding to the second PDZ
James J Matsuda et al.
American journal of physiology. Cell physiology, 294(1), C251-C262 (2007-11-06)
ClC-3 is a member of the ClC family of anion channels/transporters. Recently, the closely related proteins ClC-4 and ClC-5 were shown to be Cl(-)/H(+) antiporters (39, 44). The function of ClC-3 has been controversial. We studied anion currents in HEK293T
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