immunoprecipitation (IP): 5-10 μL using lysates of K562 cell line microarray: suitable western blot: 1:250-1:500 using mouse brain lysates western blot: 1:500-1:1,000 using K562 cells
synthetic peptide corresponding amino acids 2-18 of human CtBP1. This sequence is conserved in mouse and does not cross-react with CtBP2.
Application
Anti-CtBP1, N-terminal antibody produced in rabbit is suitable for immunoprecipitation and immunoblotting at a working dilution of 1:500-1:1000 using cell lysates of the K562 cell line and 1:250-1:500 using mouse brain lysates.
Biochem/physiol Actions
Apart from E1A protein, CtBP1 interacts with a wide variety of cellular factors such as DNA-binding transcription factors that participate in several developmental biological pathways and processes by binding to the carboxy terminal. It also interacts with polycomb group proteins and thus a component of the chromatin remodeling machinery and transcriptional repression. Pnn/DRS, a phosphoprotein involved in the regulation of cell adhesion and modulation of the activity of multiple tumor supressor genes, interacts with CtBP1 and may modulate repression of E-cadherin transcription. CtBP1 associates with SMAD6 and represses BMP signaling of the TGF-β pathway in the nucleus. It acts as transcriptional coregulator in histone acetyltransferase or histone deacetylase (HDAC)-dependent and independent manner.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
CtBP family proteins are conserved among vertebrates and invertebrates and function as transcriptional corepressors. They repress transcription in a histone deacetylase-dependent or -independent manner. CtBPs play important roles during development and oncogenesis. In this review, their unusual properties, the mechanisms
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