C8866

Checkpoint Kinase 2, Active human

buffered aqueous glycerol solution, recombinant, expressed in E. coli, ≥85% (SDS-PAGE)

• Synonym(s): S. pombe homolog, Chek2, Chk2
• MDL number: MFCD07370672
• UNSPSC Code: 12352204
• NACRES: NA.32

Properties

• recombinant: expressed in E. coli
• Quality Level: 200
• assay: ≥85% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• specific activity: ≥50 units/mg protein
• mol wt: protein apparent mol wt ~88 kDa
• UniProt accession no.: O96017
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... CHEK2(11200)

Description

Application

Kinase activity is measured as the molar amount of phosphate incorporated into the CHKtide per minute per mg protein at 30 °C using a final concentration of 50 μM [³²P] ATP.

Biochem/physiol Actions

Chk2 is the mammalian homolog of the S.cerevisiae Rad53 and S.pombe Cds1 protein kinase required for DNA damage and replication checkpoints. Chk2 is rapidly activated, by phosphorylation, in response to replication blocks and DNA damage.

Unit Definition

One unit will incorporate 1 nanomole of phosphate into CHKTIDE substrate per minute at pH 7.2 at 30 deg C.

Physical form

Supplied at a concentration of approximately 0.1 mg/mL in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable