C8413
Chloramphenicol Acetyltransferase from Escherichia coli
buffered aqueous glycerol solution
Synonym(s):
Acetyl-CoA: chloramphenicol 3-O-acetyltransferase, CAT
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
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form
buffered aqueous glycerol solution
specific activity
50,000-150,000 units/mg protein
mol wt
75 kDa (three identical subunits)
shipped in
wet ice
Storage temp.
−20°C
Gene Information
Escherichia coli ... cat(2847485)
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Application
Chloramphenicol acetyltransferase from Escherichia coli has been used in a study to assess the construction of a novel expression system in Klebsiella pneumoniae and its application for 1,3-propanediol production. Chloramphenicol acetyltransferase from Escherichia coli has also been used in a study to investigate site-directed mutagenesis and promoter functional analysis of the RM07 DNA fragment from Halobacterium halobium.
The enzyme has been used in chloramphenicol acetyltransferase assay to optimize the transfection of plasmid DNA into primary cultures of adult mouse keratinocytes.[1] It has also been used to assess the acetyl-CoA carboxylase-carboxyltransferase (ACC-CT) domain activity. This has been done using a coupled-two phase system measuring the selective partition of [14C]acetylchloramphenicol into an organic layer.[2]
Biochem/physiol Actions
The enzyme responsible for chloramphenicol resistance in bacteria. Catalyzes the conversion of acetyl-CoA + chloramphenicol to CoA + chloramphenicol 3-acetate.
Unit Definition
One unit will convert 1.0 nanomole of chloramphenicol and acetyl-CoA to chloramphenicol 3-acetate and CoA per min at pH 7.8 at 25 °C.
Physical form
Clear, colorless solution in 50% glycerol containing 5 mM Tris-HCl, pH 7.8, and 0.5 mM 2-mercaptoethanol
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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N A Betz et al.
In vitro cellular & developmental biology : journal of the Tissue Culture Association, 28A(3 Pt 1), 188-192 (1992-03-01)
An efficient and reproducible technique for the transfection of primary cultures of adult mouse keratinocytes has been developed. The procedure involves culturing the primary adult mouse epidermal cells at 32 degrees C in an enriched media until they reach 70
Hirofumi Nariya et al.
Applied and environmental microbiology, 77(23), 8439-8441 (2011-10-04)
A xylose-inducible gene expression vector for Clostridium perfringens was developed. Plasmid pXCH contains a chromosomal region from Clostridium difficile (xylR-P(xy)(lB)): xylR, encoding the xylose repressor, xylO, the xyl operator sequence, and P(xylB), the divergent promoter upstream of xylBA encoding xylulo
Vanessa Fuentes et al.
Eukaryotic cell, 11(6), 725-734 (2012-04-03)
Synthesis of functional mRNA in eukaryotes involves processing of precursor transcripts, including the addition of a poly(A) tail at the 3' end. A multiprotein complex recognizes a polyadenylation signal, generally the hexanucleotide AAUAAA in metazoans, to direct processing of the
Ziyaad Valley-Omar et al.
Virology journal, 8, 462-462 (2011-10-07)
HIV-1 Gag virus like particles (VLPs) used as candidate vaccines are regarded as inert particles as they contain no replicative nucleic acid, although they do encapsidate cellular RNAs. During HIV-1 Gag VLP production in baculovirus-based expression systems, VLPs incorporate the
Kai Zhou et al.
Research in microbiology, 163(5), 316-322 (2012-06-05)
An intragenic tandem repeat (TR) region has been previously reported in the tolA gene of Escherichia coli. In silico analysis of 123 E. coli tolA sequences from Genbank and PCR analysis of the tolA TR region from 111 additional E.
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