C7365
Anti-phospho-c-Raf (pSer338/pTyr340) antibody produced in rabbit
affinity isolated antibody, buffered aqueous glycerol solution
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About This Item
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous glycerol solution
species reactivity
mouse, rat, human
technique(s)
western blot: 1:1,000 using cell lysates from Hek293 cells transfected with c-Raf, or co-transfected with
c-Raf, Ras[V12] and Rac1.
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
phosphorylation (pSer338/pTyr340)
Gene Information
human ... RAF1(5894)
mouse ... Zhx2(387609)
General description
c-Raf or Raf-1 belongs to the Raf family of kinases that have regulatory functions in cell growth, differentiation, growth factor receptor stimulation via the MAPK signalling. The other members of the family are A-Raf and B-Raf. All the isoforms have unique as well as overlapping regulatory functions. c-Raf kinase is the first identified downstream effector kinase of Ras, and is the activator of MEK which in turn activates ERK. Phosphorylation of c-Raf at Ser338 and Tyr340 regulates its biological function.
Anti-phospho-c-Raf [pSer338/pTyr340] detects human c-Raf [pSer338/pTyr340]hosphorylated at [pSer338/pTyr340]er338 and [pSer338/pTyr340]yr340 (74 kDa). Mouse and [pSer338/pTyr340]at c-Raf (100% homology) are [pSer338/pTyr340]xpected [pSer338/pTyr340]o cross react.
Anti-phospho-c-Raf [pSer338/pTyr340] detects human c-Raf [pSer338/pTyr340]hosphorylated at [pSer338/pTyr340]er338 and [pSer338/pTyr340]yr340 (74 kDa). Mouse and [pSer338/pTyr340]at c-Raf (100% homology) are [pSer338/pTyr340]xpected [pSer338/pTyr340]o cross react.
Immunogen
synthetic phosphopeptide derived from the region of human c-Raf that contains serine 338 and tyrosine 340.
Application
The antibody may be used to detect phospho-c-Raf (Ser338/Tyr340) by immunoblotting at working dilution of 1:1000 in cell lysates from HEK293 cells transfected with c-Raf. Detection was also possible in spermatozoa of mice at 1:1000 dilution.
Physical form
Solution in Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, with 50% glycerol, 1.0 mg/mL BSA (IgG and protease free) and 0.05% sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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B H Zhang et al.
Experimental lung research, 27(3), 269-295 (2001-04-11)
The Raf serine/threonine kinase plays an essential role to relay intracellular signals from the protooncogene Ras to activation of the mitogen-activated protein kinase (MAPK) cascade. The Raf kinase family consists of C-Raf (Raf-1), B-Raf, and A-Raf. Extensive efforts have been
J T Lee et al.
Leukemia, 16(4), 486-507 (2002-04-18)
The Raf/MEK/ERK (MAPK) signal transduction cascade is a vital mediator of a number of cellular fates including growth, proliferation and survival, among others. The focus of this review centers on the MAPK signal transduction pathway, its mechanisms of activation, downstream
E O'Neill et al.
British journal of cancer, 90(2), 283-288 (2004-01-22)
The Raf-MEK-ERK signalling pathway controls fundamental cellular processes including proliferation, differentiation and survival. It remains enigmatic how this pathway can reliably convert a myriad of extracellular stimuli in specific biological responses. Recent results have shown that the Raf family isoforms
Brett Nixon et al.
Journal of cellular physiology, 224(1), 71-83 (2010-03-17)
Recent studies from within our laboratory have demonstrated a causal relationship between capacitation-associated surface phosphotyrosine expression and the ability of mouse spermatozoa to recognize the oocyte and engage in sperm-zona pellucida interaction. In the studies described herein we have sought
B Diaz et al.
Molecular and cellular biology, 17(8), 4509-4516 (1997-08-01)
Activation of the Raf serine/threonine protein kinases is tightly regulated by multiple phosphorylation events. Phosphorylation of either tyrosine 340 or 341 in the catalytic domain of Raf-1 has been previously shown to induce the ability of the protein kinase to
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